Choose from 500 different sets of streak plate method flashcards on quizlet. Nov 25, 2017 the following methods are used to isolate pure culture. This action is repeated for each of the four quadrants of the plate. Microbes in action this material may be duplicated by teachers for use in the classroom. Streaking is a method that isolates a pure strain from a species of bacteria. Streaking microbial cultures on agar plates, page 2 1999 science in the real world. Dip the loop into the broth culture containing the mixture of bacteria. In microbiology, streaking is a technique used to isolate a pure strain from a single species of microorganism, often bacteria. Isolation of bacterial colonies using the quadrant method the streak plate procedure is designed to isolate pure cultures of bacteria, or colonies, from mixed populations. What is critical is the generation of isolated colonies. Home basic microbiology streak plate method principle, methods, significance, limitations. See the instructional photo that appears with this article.
A presterilized loop, stick or toothpick is used to spread the sample across onequarter of the agar surface with a rapid, smooth, backandfourth motion from the rim to the center of the plate. The inoculum is streaked over the agar surface in such a way that it thins out the bacteria. Streak plate technique for isolation of microorganism. The spread plate technique involves using a sterilized spreader with a smooth surface made of metal or glass to apply a small amount of bacteria. Method of isolation of pure culture linkedin slideshare. Aug 15, 2019 spread plate technique is the method of isolation and enumeration of microorganisms in a mixed culture and distributing it evenly.
The techniques commonly used for isolation of discrete colonies initially require that the number of organisms in the inoculums be reduced. Samples can then be taken from the resulting colonies and a. The serial dilution method is utilized to decline the number of microorganism in the inoculum. The aim of the procedure is to obtain single isolated pure colonies. The technique makes it easier to quantify bacteria in a solution. The inoculum is streaked over the agar surface to isolate colonies on at least a portion of the plate. As well as causing a familiar range of diseases in animals and plants and problems in food spoilage and deterioration of other materials, microbes are also our invisible allies. Pouring a plate 6 storage of media 6 sterilisation vs disinfection 6 sterilisation using the autoclavepressure cooker 7 sterilisation of equipment and materials 7 choice, preparation and use of disinfectants 7 inoculation and other aseptic procedures essential points 8 using a wire loop 8 using a pipette 9 flaming the neck of bottles and test. The streakplate procedure is designed to isolate pure cultures of bacteria, or colonies, from mixed populations by simple mechanical separation. In essence, the streak plate technique is a type of dilution. A template for the streak plate isolation technique for laboratory classrooms. Pour plate technique for bacterial enumeration david b. This method is used for isolating the discrete colonies of the microorganism.
To insure a countable plate a series of dilutions should be plated. Streak plate technique is used for the isolation into pure culture of the organisms mostly bacteria, from mixed population. After the loop has cooled, in one of the quadrants left, run your loop into the first region three times, then streak the quadrant. Most microbiologists repeat streaking from an isolated colony two to three times before they guarantee culture purity. Start studying 15 exercise streak plate methods of isolation. If more than one shape or colour of colony on the streak lines is evident, this indicates that a culture contains more than one type or species of bacterium or yeast. The streak plate technique is the most popular method for isolating specific bacteria from a sample containing a mixture of microorganisms. Isolate single bacterial colonies by the streakplating method.
Feb 04, 2016 why cant your body handle a punch to the liver. A common method for the isolation of a pure culture from a mixture is by streaking plates. Label the bottom of the plate with the above data plus initials, seat number and date. Spread plate technique methods of isolating pure culture 3. The microbial mixture is seeded on the edge of an agar plate with inoculating loop and than streaked out over the surface. Streak plate virtual interactive bacteriology laboratory. Turn the plate 90, overlap the previous area 12 times, and streak into the next quadrant as in step 4. By using streak plate technique to spread a clinical sample out on the surface of a solid growth medium individual types of bacteria can be isolated. To obtain a pure culture, it is required to obtain separate, single bacterial colony. May 11, 2012 a streak plate technique using the quadrant method. It is essentially a dilution technique that involves spreading a loopful of culture over the surface of an agar plate. For the other tsa plate, using the above procedure as a guide, perform the streak plate using the fourphase method. Streak plate method principle, methods, significance, limitations.
Van soestbergen and chingholee department of pathology, college of medicine, ohio state university, columbus, ohio 43210 receivedfor publication 12 september 1969 thepourplate methodofcountingbacteria is moreprecise than the streak plate method, but, onthe average, it will give alower count. Streaking microbial cultures on agar plates science buddies. Principle the streak plate technique is essentially a method to dilute the number of organisms, decreasing the density. Basic practical microbiology why microbiology matters. The main difference shall be how far the plate is turned during each streak. Sterilize the inoculating loop in the bunsen burner by clicking on the loop and dragging it to the burner. However, to isolate colonies, some folks prefer the threephase streak plate technique, whereas others prefer the fourquadrant fourphase technique.
The method most often used in the clinical laboratory is the quadrant method. This is to make sure that the individual cells fall apart on the agar medium surface so as. Streak plate method principle, methods, significance. The most common method of separating bacteria cell on agar surface to obtain isolated colonies is the streak method to inoculate petri dish. Spread plate technique principle, procedure and uses. Transfer to the growth media as quickly as possible. The streak plate method is recommended for isolating individual bacterial or fungal colonies. Full text get a printable copy pdf file of the complete article 209k, or click on a page image below to browse page by page. Inoculation of agar plates using a swab a sterile swab must be used, care must be taken not to contaminate by touching other surfaces. A disadvantage of the streak plate technique could be colony isolation problems. The inoculum is streaked over the agar surface in such a. One very common way to isolate bacterial and other microbes is by employing the streak plate technique.
T streak methodd streak one half of your agar plate with the bacteria, then flame your loop. Ada beberapa cara umum yang dapat dilakukan untuk mengisolasi mikroba antara lain, untuk mengisolasi bakteri dapat dilakukan dengan cara goresan streak plate, cara taburan atau tuang pour palte, cara sebar spread plate, cara pengenceran dilution method, serta mikromanipulator the micromanipulator method lim, 1990. Thanks a lot your website is very useful for me i was able to make my own notes and easy to understand. Use pourplating and spreadplating methods to determine the concentration of. There are alternative methods for preparing a streak plate but the method shown is. Nov 11, 2018 in microbiology, streaking is a technique used to isolate a pure strain from a single species of microorganism, often bacteria. The procedure includes a dilution technique which requires spreading a loopful of culture over the agar plate surface. Study the diagram and read the tips below the diagram before you begin the streak plate. Streak plate method of isolation purpose the streak plate technique is the most widely used method of obtaining isolated colonies from a mix of cultures. A template for the streak plate isolation technique for. What is a disadvantage of the streak plate technique. This plate illustrates a streak plate which did not achieve isolation, and which would not be considered a good streak plate example. Samples can then be taken from the resulting colonies and a microbiological culture can be grown on a new plate so that the organism can be identified, studied, or tested. Disadvantage of the streak plate technique answers.
Streak the plate, following either the t method or quadrant streak shown below. A sample is taken from a colony and a microbiological culture is grown on the new plate in order for the organism to be identified properly. The streak plate method requires the number of organisms in the inoculums be reduced. Ideal results require that only a small amount of inoculum be used and that the surface of the plate be free of droplets of condensed. Learn streak plate method with free interactive flashcards. The streak plate culture technique for the isolation of microorganism is the most practical method of obtaining discrete and welldeveloped colonies of the microbe in pure cultures. Streak plate technique is used for the isolation into a pure culture of the organisms mostly bacteria, from a mixed population. This uses either the three streak or four streak methods. In streak plate culture method, a sterilized loop or transfer needle is dipped into the mixed culture of the specimen. Welcome to microbugz streak plate method of isolation. To attain this, well known quadrant streaking method can be used. The technique essentially dilutes the number of organisms and reduces their density. Please available all notes in pdf formet also so we can download. As you might guess, the purpose of streaking for isolation is to produce isolated colonies of an organism on an agar plate.
Described below is the zigzag method of inoculation. If the streaking technique is not done properly or if there is too much of an organism present on the inoculating. Learn vocabulary, terms, and more with flashcards, games, and other study tools. The streak plate method is a rapid qualitative isolation method. The exact streak plate method that one uses to generate isolated colonies is not too critical. Some individual bacterial cells are separated and well spaced from each other. It can be used when starting a microbiologics product such as a kwikstik or when simply subculturing a colony from. This allows for individual colonies to be isolated from other colonies. Streak plate technique streaking is the process of spreading the microbial culture with an inoculating needle on the surface of the media. The pour plate method of counting bacteria is more precise than the streak plate method, but, on the average, it will give a lower count. The dilution or isolation by streaking method was first developed by loeffler and gaffky in kochs laboratory, which.
The streak plate method is a rapid qualitative isolation method for obtaining discrete colonies from a mixed population. Streak plate technique is used to grow bacteria on a growth media surface so that individual bacterial colonies are isolated and sampled. Depending on your purposes, you can inoculate the swab in a few ways. An introduction to microbiology, aseptic technique and safety.
381 432 998 615 1345 1416 486 689 386 209 829 366 1653 1433 858 1427 721 640 48 1530 233 1591 1519 1237 175 784 36 55 1073 795 922